Table 1.
Tissue | Active MMP-2 Activity | Pro-MMP-2 Activity | Pro-MMP9 Activity | ||||||
---|---|---|---|---|---|---|---|---|---|
SDU | TPH μU | TLU | SDU | SPSμU | TLU | SDU | SPSμiU | TLU | |
Normal (n=30) | 14.2 | 532.5 | 19.2×103 | 147.6 | 0.554 | 704.1×103 | 190.8 | 24.2 | 1640.9×103 |
Ad w/o HGD (n=18) | 31.8 | 1,192.5 | 43.0 ×103 | 172.0 | 0.645 | 820.4×103 | 337.3 | 42.8 | 2900.8×103 |
Ad with HGD (n=16) | 63.3 | 2,373.8 | 85.7 ×x103 | 178.5 | 0.669 | 851.5×103 | 581.3 | 73.8 | 4999.2×103 |
Cancer (n=12) | 127.0 | 4,762.5 | 171.8 ×103 | 264.3 | 0.991 | 1260.7x103 | 726.7 | 87.2 | 6249.6×103 |
*SDU, serial dilution units (defined by serial dilution of sample required to detect minimal complete visible band); TPH (μU, thiopeptolide hydrolysis activity in microunits; SPS μU, synthetic peptide hydrolysis activity in microunits against the dinitrophenyl-linked synthetic peptide substrate 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH; TLU, Total Lab units, computer imaging units in band area × pixel intensity as defined by Nonlinear Dynamics Total Lab software.