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. 2011 Feb 3;25(4):669–680. doi: 10.1210/me.2010-0437

Fig. 7.

Fig. 7.

Activation of CamKII is necessary and sufficient for induction of c-Fos. A, To confirm the specificity of the KN-93 inhibitor, we cotransfected a dominant-negative CamKII (dnCamK) or treated the LβT2 cells with the inhibitor of CamKK, STO-609, and treated with vehicle (white bars) or GnRH (black bars). *, Significant decrease in fold induction in the cells cotransfected with the dominant-negative CamKII. B, Western blot of the whole-cell lysates treated with GnRH or cotransfected with constitutively active CamKII (caCamK), and probed with antibodies for phospho-SRF and total SRF. C, LβT2 cells were cotransfected with the wild-type (WT) c-Fos reporter or with c-Fos containing a mutation in the SRF-binding site (mutSRF), and constitutively active CamKII (caCamK; black bars) or vector control (white bars). *, Significant induction by caCamK, not observed in the cells cotransfected with c-Fos reporter containing a mutation in the SRF-binding site. ctrl, Control.