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GH induces occupancy of phosphorylated C/EBPβ and p300 on promoters of multiple early response genes. 3T3-F442A preadipocytes were treated without (−) or with (+) GH for 30 min. Nuclear extracts were analyzed by ChIP using antibodies against p300, P-C/EBPβ, or C/EBPβ. Controls contain no antibody; 1% input is also shown. ChIP samples were analyzed with probes specific to the known or predicted C/EBP-CREB sites in the c-Fos, Cyr61, Btg2, and Socs3 promoters.
