Table 1.
GH-induced early response genes
| Gene symbol | GH/Con | C/EBP-CREB |
|---|---|---|
| Socs3 | 17.47 | Yes |
| Fos | 4.39 | Yes |
| Egr2 | 3.66 | Yes |
| Cyr61 | 2.28 | Yes |
| Egr1 | 2.19 | Yes |
| Junb | 2.14 | Yes |
| Btg2 | 2.13 | Yes |
| Cited2 | 1.89 | Yes |
| Frat2 | 1.83 | Yes |
| Il6 | 1.66 | Yes |
| Klf4 | 1.50 | Yes |
| Phlda1 | 5.32 | No |
| Socs1 | 2.82 | No |
| Zfp36 | 2.68 | No |
| Ier2 | 2.41 | No |
Based on microarray analysis (19), the expression of the genes listed was stimulated by GH at least 150% above control (GH/Con) in 3T3-F442A adipocytes. They were present in a cluster of genes stimulated by GH at 30 min but not at 4 or 48 h. Sequence analysis was performed as described in Materials and Methods to identify predicted C/EBP-CREB motifs that were conserved in both mouse and human gene promoters (−1500 to +200).