Fig. 1.

T stimulates Cyp19 and P450scc expression. A, Granulosa cells were cultured in the presence or absence of increasing concentrations of T for 96 h. Cyp19, P450scc, and β-actin (BACT) protein expression was determined by Western blot. Similar results were obtained in three independent experiments. B and C, Granulosa cells were cultured in the presence or absence of T (100 nm) for 48 and 96 h. Cyp19 and P450scc expression was determined by quantitative PCR and expressed as relative expression to ribosomal L19 protein. *P < 0.05, ** P < 0.001 vs. 0. D, Cyp19 mRNA levels were determined by Northern blot analysis after incubation of granulosa cells with vehicle (C) or T (100 nm). E, E₂ production was determined by ELISA analysis in cells culture with vehicle (C) or T (100 nm); *** P < 0.001 vs. C.