Fig. 3.

Effect of different steroids on the expression of Cyp19 and P450scc. Cells were cultured in the presence of different steroids (100 nm) for 96 h. Cyp19 (A) and P450scc (B) expression was assessed by real-time PCR. This experiment was performed five times with identical results. Values are the mean ± sem of a single experiment performed in triplicate. C, Granulosa cells were cultured in the presence of increasing concentrations (20, 100, or 500 nm) of T, Δ4A, or DHT. Cyp19 expression was determined using real-time PCR. Columns denoted with different letters differ significantly (a and b, P < 0.05; a–d and a–c, P < 0.001; ANOVA I Tukey t test). E1, Estrone.