Fig. 5.

T stimulation of Cyp19 and P450scc expression is mediated by the AR. A–C, Granulosa cells were treated with vehicle, T, or Δ4A in the presence or absence of hydroflutamine (HF) or bicalutamide (CX), which are two antagonists of AR or in the presence of fulvestrant (ICI), which is an ER antagonist. D and E, Granulosa cells were infected with lentivirus carrying shRNA sequences against luciferase (shLUC), used as control, or two regions of the AR-coding sequence (shAR 1 and 2). Cyp19 and P450scc mRNA levels were quantified by real-time PCR. F, AR protein expression after infection with shLuc or shAR2. These experiments were performed three times with identical results. Values are the mean ± sem of a single experiment performed in triplicate. Columns denoted with different letters differ significantly (a–c, P < 0.05; a and b, P < 0.001; ***, P < 0.01 vs. control or Δ4A plus CX; ANOVA I Tukey t test).