Fig. 6.

T stimulates Cyp19 promoter activity. A, Granulosa cells were transfected with luciferase reporter constructs containing serial deletions of the Cyp19 proximal promoter: 1100, 600, or 245 bp. B, Granulosa cells were transfected with the 245-Cyp19-luc reporter construct containing no mutations [wild type (WT)], or mutations on the following response elements: NRHS (mNRHS), cAMP-responsive element-like sequence (mCLS), or AP-3 (mAP3). Luciferase activity was determined 48 h after transfection. Results from four experiments performed in triplicate are shown as the mean ± sem. Columns with asterisks (**) differ significantly from control (ANOVA-I Tukey t test).