Fig. 8.

T induces LRH-1 expression and LRH-1 binding to the Cyp19 promoter. A, LRH-1 expression after treatment of granulosa cells with vehicle or T. B, EMSAs were performed with nuclear extracts of granulosa cells cultured in the presence or absence of T for 96 h and probes containing the NRHS or CLS elements found in the Cyp19 promoter. C, Chromatin was immunoprecipitated with LRH-1 antibodies or normal IgG. Immunoprecipitated (ChIP) or total (Input) DNA was amplified by regular PCR (left) or by real-time PCR (right) using primers that amplified the NRHS region of the Cyp19 proximal promoter (PII). As control, exon 9 (Ex9) of the coding region was also amplified. These experiments were performed four times with identical results. Values are the mean ± sem of a single experiment performed in triplicate. ***, P < 0.01 vs. control Student's t test.