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Lacunar microstructure in the 13-lined ground squirrel femur. Lacunae in the plane of focus (white arrows) were used for quantification of lacunar properties including average osteocyte lacunar area [Ot.Lc.Ar, measured as the area (μm2) inside the lacunar border], osteocyte lacunar density (Ot.Lc.Dn, measured as the total number of lacunae per total bone area; μm2) and osteocyte lacunar porosity (Ot.Lc.Po, measured as the percentage total lacunar area divided by total bone area).
