Table 1.

Definitions of shorthand for protocols used in this study

Designation	Color	Storage	Perfusion	Electrode
L-15/Locke's	Red	L-15 culture media on ice	Locke's, 36°C	HEPES Locke's, 36°C
Locke's/Locke's	Blue	Locke's, 32°C	Locke's, 36°C	HEPES Locke's, 36°C
Ames'/Ames'	Black	Bicarb. Ames, 32°C 1.15 mm Ca2+, 1.2 mm Mg2+	Bicarb. Ames, 36°C 1.15 mm Ca2+, 1.2 m Mg2+	HEPES Ames, 36°C 1.15 mm Ca2+, 1.2 mm Mg2+
Ames'Ca,Mg/Ames'Ca,Mg	Green	Bicarb. Ames, 32°C 1.2 mm Ca2+, 2.4 mm Mg2+	Bicarb. Ames, 36°C 1.2 mm Ca2+, 2.4 mm Mg2+	HEPES Ames, 36°C 1.2 mm Ca2+, 2.4 mm Mg2+
Ames'Mg /Ames'Mg	Magenta	Bicarb. Ames, 32°C 1.15 mm Ca2+, 2.4 mm Mg2+	Bicarb. Ames, 36°C 1.15 mm Ca2+, 2.4 mm Mg2+	HEPES Ames, 36°C 1.15 mm Ca2+, 2.4 mm Mg2+

“Designation” denotes storage and perfusion solutions in each protocol. The color scheme defined here is held constant throughout the figures.