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. 2011 Mar 21;192(6):993–1004. doi: 10.1083/jcb.201011111

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Figure 1. — Differential response of TC-32 cells to dilutions of Wnt-1 and Wnt-3a CM. After 3 h incubation, whole-cell lysates were immunoblotted with antibodies to Dvl-2, Dvl-3, and HSP70, the last serving as a loading control. Control cells (Ctl.) were incubated with serum-free culture medium for 3 h before processing. Arrows highlight doublet bands indicative of phosphorylation.