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. 2011 Mar 21;192(6):979–992. doi: 10.1083/jcb.201005100

Figure 1.

Figure 1.

Effects of plasma membrane PS on membrane translocation of Akt-PH domain and Akt activation. (a) Increasing plasma membrane PS in Neuro 2A cells by DHA supplementation facilitated IGF-induced Akt membrane translocation and phosphorylation. The membrane translocation is depicted by a representative fluorescence intensity profile across a transverse section in indicated cells (FI). (b) Decreasing plasma membrane PS using PSA-3 mutant CHO cells impaired Akt-PH domain translocation and Akt phosphorylation without affecting PI3 kinase activity. Shown are representative micrographs, time course of translocation by using averaged relative R values, and PI3 kinase activity. (c) Increasing plasma membrane PS by expressing the PSS1(R95K) facilitated IGF-induced Akt activation without altering PI3 kinase activity in Neuro 2A cells. GFP-PSS1 was observed at the apparent molecular mass of ~68 kD. The dimeric form of GFP (∼52 kD) was detected in samples expressing GFP alone. The plasma membrane PS contents are expressed as mean ± SD (error bars; n = 3), representing two independent experiments. *, P < 0.05. Bars, 10 µm.