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. 2011 Mar 21;192(6):979–992. doi: 10.1083/jcb.201005100

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Figure 2. — Effects of membrane phospholipids on interdomain conformational changes of Akt due to membrane interaction probed by mass spectrometry. The appearance of two interdomain cross-linked peptides (K30-K389 and K284-K426) depended on the phospholipid composition of interacting membranes. Cross-linked peptides are marked with asterisks (left). The zoomed-in-views (middle) show quantitative comparison of these cross-linked peptides using ¹⁶O/¹⁸O labeling as described in Materials and methods. Although the¹⁶O/¹⁸O ratio from non–cross-linked peptide pairs such as T[87–111]K (2886 D vs. 2990 D) remained the same, the ¹⁶O/¹⁸O ratio from the interdomain cross-linked peptide pairs separated by 8 D changed according to the membrane phospholipid composition. Schematic presentations of the interdomain conformational changes of Akt deduced by the cross-linking data are shown (right).