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. 2011 Mar 23;6(3):e17968. doi: 10.1371/journal.pone.0017968

Table 1. Bacterial distribution on the ‘EC’SFS and NFS slopes.

EC South Facing Slope EC North Facing Slope
S Sh S Sh
Plant material1 Bulk soil1 Plant material Bulk soil Plant material Bulk soil Plant material Bulk soil
Total bacterial count1 106±0.4×106 104±0.2×104 106±0.4×106 104±0.3×104 106±0.3×106 104±0.5×104 107±0.2×107 104±0.2×104
Spore ratio to vegetative cells2 (%) 0.5 55 0.6 60 0.7 7 0.5 12
ACC utilization3 (%) 50 1 52 1 5 1 3 1
Biofilm formation4 (%) 55 2 50 1 4 1 5 1
Halophilic growth5 (%) 51 1 55 1 5 1 4 1
P solubilization6 (%) 50 2 49 2 3 1 2 2
1

The data are expressed per gram of fresh weight. Wild barley roots were prepared as described in Material and Methods.

Each data point represents three independent experiments. The culturable aerobic fraction of the total bacterial CFUs was determined on TSA plates as described in Material and Methods s-sun area; sh-shaded area.

2

Spores were counted using Shaeffer-Fulton staining.

3

ACC (1-Aminocyclopropane-1-carboxylate) medium was composed of salts for P. polymyxa minimal medium there the nitrogen source is replaced with ACC.

4

Biofilm formation was estimated as described in Material and Methods. The isolates with OD >1 were considered as biofilm formers.

5

Halophilic growth was determined in the medium containing 2 M NaCl (see Material and Methods).

6

P solubilization was estimated using NPRJP medium (see Material and Methods). The colonies with index >2 were considered as P solubilizers.