Table 1. Recovery for all sample types.
Extraction method | |||||||
modified | modified | ||||||
Sample type | Griffiths 0.5 g | Powersoil™ 0.5 g | Ultraclean™ 0.5 g | FastDNA® Spin kit 0.5 g | QIAamp Stool kit 0.5 g | Griffiths 0.1 g | FastDNA® Spin Kit 0.1 g |
Badger faeces | 0.00 (0.00–0.00) | 0.00 (0.00–0.00) | 0.00 (0.00–0.00) | 0.06 (0.00–0.12) | 0.00 (0.00–0.00) | 10.87 (5.73–14.67) | 21.48 (13.94–48.82) |
Badger faeces (10× diluted) | 0.05 (0.00–0.12) | 0.00 (0.00–0.00) | 0.00 (0.00–0.00) | 0.00 (0.00–0.05) | 0.00 (0.00–0.00) | n.a. | n.a. |
Cow slurry | 0.19 (0.13–0.28) | 0.00 (0.00–0.01) | 0.00 (0.00–0.00) | 0.07 (0.00–0.17) | 0.00 (0.00–0.00) | n.a. | 11.48 (8.72–23.43) |
Kilkenny soil | 18.72 (9.13–63.26) | 23.92 (8.45–32.65) | 1.55 (0.8–4.41) | 85.04 (30.25–100) | 12.53 (11.13–16.11) | n.a. | 51.05 (16.12–62.72) |
Stockton soil | 6.97 (3.37–21.42) | 8.82 (4.58–9.93) | 0.89 (0.58–1.8) | 23.08 (10.51–30.2) | 1.86 (0.68–3.95) | n.a. | 30.79 (6.57–43.96) |
Warwick soil | 16.23 (6.7–21.28) | 18.07 (10.3–26.93) | 10.51 (5.83–18.8) | 79.79 (49.31–100) | 9.99 (7.89–12.03) | 2.91 (0.32–9.59) | 49.16 (27.58–73.54) |
The recovery (percentage) shown is the median value of the top 4 spikes, interquartile range values are presented in brackets. n.a.: not available.