Table 2. Mass spectrometric analysis of AP-1γ IPs: relative performance of soft vs standard elution.

Protein	Mascot Scores Soft/Standard	Peptide Count Soft/Standard
AP-1β	1.25	1.15
AP-1µ1a	0.96	0.92
AP-1σ1a	1.21	1.27
Aftiphilin	1.58	1.66
γ-Synergin	2.00	1.45
p200	1.21	1.45
KIF13a	1.51	1.48
Average ± SD	1.39±0.34	1.34±0.25

For a meaningful comparison only the seven most abundant co-precipitants of AP-1γ were included (see Table 1). Ratios are the averages of three independent experiments.