Figure 2.
The autoproteolyzed products of Nup98-6kDa and Nup98-Nup96 remain associated with each other. Several mRNAs coding for myc-tagged Nup98 variants were translated in a reticulocyte lysate system. Total translation products (lane 3, expression) or immunoprecipitates of translation products with anti-myc antibodies (lanes 1, 2, and 4) were analyzed by SDS/PAGE (4–20% in A and 8% in B) and autoradiography.