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TPR binds to Nup98 but not to Nup96. Several mRNAs coding for myc-tagged TPR or indicated Nup98 variants were translated separately in a reticulocyte lysate system. As indicated, aliquots were analyzed either directly (expression) or after posttranslational incubation and immunoprecipitation with the indicated antibodies (anti-Nup98 or anti-Nup96) or mock-immunoprecipitation in the absence of antibodies (control). Analysis was by SDS/PAGE and autoradiography.
