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Nup96 binds to TPR indirectly via Nup98. Several mRNAs coding for myc-tagged TPR or Nup98 variants were translated separately in a reticulocyte lysate system. As indicated, aliquots were analyzed either directly (expression) or after posttranslational incubation and immunoprecipitation with the indicated antibodies. Analysis was by SDS/PAGE and autoradiography.
