Table 1.
Details of primers used for real-time PCR analysis
| Gene | GenBank Accession Number | Forward Primer (5'-3') |
Reverse Primer (5'-3') |
|---|---|---|---|
| 18S | NR_003286 | TTCGGACGTCTGCCCTATCAA | ATGGTAGGCACGGCGACTA |
| α-actin | NM_001100 | GTAGCTAACCGCCCAGAAACT | AGGCCGGAGCCATTGTC |
| CyclinD1 | NM_053056 | GCATGTTCGTGGCCTCTAAGA | CGGTGTAGATGCACAGCTTCTC |
| eMHC | NM_002470 | TCCTGGCTGTTGCTGTCTTCT | ACTTCCATTTCAGTGTCACTACTCATG |
| MyoD | NM_002478 | CCGCCTGAGCAAAGTAAATGA | GCAACCGCTGGTTTGGATT |
| Myogenin | NM_002479 | GGTGCCCAGCGAATG | TGATGCTGTCCACGATCGA |
| SOCS3 | NM_003955 | GACCAGCGCCACTTCTTCA | CTGGATGCGCAGGTTCTTG |
Primer sequences were designed using Primer Express version 3.0 software (Applied Biosystems) using sequences accessed through GenBank and checked for specificity using Nucleotide-Nucleotide Blast search. Embryonic myosin heavy chain, eMHC.