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. 2011 Mar 8;12:19. doi: 10.1186/1471-2172-12-19

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Copyright ©2011 Khan et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of serum autoantibodies against both native and modified GAD₆₅in all subjects. Direct binding ELISA of 1:100 diluted uncomplicated and complicated (nephropathy and retinopathy) T1D serum samples. Control samples were served as controls. Serum autoantibodies reactivity of all four groups was checked towards N-GAD₆₅(□) and ROS-GAD₆₅(■). Microtitre plates were individually coated with N-GAD₆₅and ROS-GAD₆₅(20 μg/ml). Each histogram represents the mean ± SD values.