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. 2011 Mar 23;6(3):e18027. doi: 10.1371/journal.pone.0018027

Figure 4. Enhanced antigen sensitivity mediated by a G107S substitution.

Figure 4

(A) The 1MOG9 wt or G107S TCR were transduced into 4G4 or 4G4.CD4 TCRαβ-deficient hybridoma cells. The cell lines were flow cytometrically sorted for equivalent TCR expression and stimulated with MOG35–55 peptide or αCD3 and irradiated C57BL/6 or class II−/− splenic APCs as indicated. IL-2 production was measured at 24 h. Similar studies were performed with 4G4.CD4 cells transduced with wt or G107S substituted 1MOG244.2 (B), PA-19 (C), and 3A9 TCR (D).