Figure 6. Fine specificity of wt and G107S TCR.

To determine if the G107S substitution influenced fine specificity, 1MOG9 (A) or 1MOG244.2 (B) transduced 4G4.CD4 cells or their corresponding G107S mutants were stimulated with 10 or 100 µg/ml of the indicated A-substituted MOG_35–55 peptides. IL-2 production, normalized to the response to wt MOG_35–55, is plotted. Plots are representative of 3 independent studies performed with 10 µg/ml and 2 with 100 µg/ml peptide Ag. *, p<0.05 by ANOVA.