Skip to main content
. 2011 Mar 23;6(3):e18114. doi: 10.1371/journal.pone.0018114

Figure 4. CRF-induced CREB phosphorylation occurs via a MEK/MAPK-dependent mechanism.

Figure 4

(A) The CaMK inhibitor KN-93 (2 µM) did not affect CRF-induced CREB phosphorylation (F = 17.65). (B) CRF-induced CREB phosphorylation was blocked by the MEK antagonist U0126 (10 µM; F = 11.97). (C) The L-type Ca2+ channel blocker nifedipine (5 µM) had no effect on CRF-induced CREB phosphorylation (F = 18.50). (D) Chelating intracellular calcium with BAPTA-AM (30 min incubation with 5 µM) failed to block CRF-induced CREB phosphorylation (F = 12.72).