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Time line of the experimental protocol. On day 0, cells are seeded onto the polymer. Then, 3–7 days later, the polymer graft is implanted on the right ovarian pedicle. On day 10–14, the IGROV-1 cell clot is implanted under the left renal capsule. Then, 2–3 wk later, the tumor sizes are measured. The kidney and the ovarian pedicle are removed for immunohistochemical and/or histologic analysis. Serum is collected throughout the protocol to measure MIS.
