Figure 2.

(A) MIS in serum is influenced by polymer implant size. Polymer squares were seeded with MIS-producing cells 3 days before implantation. After 21 days, marked differences in mean MIS serum (n = 4 per polymer size) were seen in the animals implanted with polymers of varying sizes. Then, 0.5 cm² grafts were used for further study. (B) Increasing serum MIS was detected on days 7, 14, 21, and 28 in SCID mice after the CHO-B9 seeded polymer was implanted. This composite figure shows the mean +/− the SEM for 40 animals. (C) The accumulation of MIS in the serum of polymer-bearing mice is reversible. Two weeks after implantation, serum MIS was approached 1200 ng/ml. Upon polymer removal, MIS falls to undetectable levels; thus there is no migration of cells from the implantation site. This figure is representative of six experiments.