Figure 5.

Monocyte Subset-Specific Activation Pathways

(A) Phosphorylated Akt, p38-MAPK, MEK1, and JNK in CD14⁺(black bars) and CD14^lo monocytes (red bars) following treatment with TLR8 agonist (3M2, open bars) or TLR7 agonist (3M13, closed bars) for 0, 30, and 120 min at 37°C. Data are presented as mean fold increase from vehicle control treated cells from three independent experiments.

(B) Cytokine production by CD14⁺ (open black bars) and CD14^dim (closed red bars) monocytes treated with vehicle, 3M2, or 3M13 with or without MEK inhibitor PD98059. Data are presented as mean ± SD in expression (pg/ml) from three independent experiments.

See also Figure S4.