Fig. 2.

Caspase-mediated JIP1 cleavage during staurosporine induced apoptosis of HeLa Cells. HeLa cells were treated with staurosporine (1 μM) with or without 50 μM zVAD.fmk (added 1 h prior to the addition of staurosporine) for the times indicated. Adherent and non-adherent cells were collected, the percentage of apoptotic cells determined, and cell lysates immunoblotted with either monoclonal antibodies to JIP1, PARP and α-tubulin (A) or polyclonal antibodies raised against caspases-3, -7, -8 and -9 (B).