Fig. 3.

Effect of caspase inhibitors on JIP1 cleavage during TRAIL-induced apoptosis of HeLa cells. HeLa cells were treated with TRAIL (1 μg/ml) for 8 h in the presence or absence of either 50 μM zVAD.fmk or DEVD.fmk at the concentrations indicated. Caspase inhibitors were added 1 h prior to the addition of TRAIL. Adherent and non-adherent cells were collected and the percentage of apoptotic cells determined and lysates immunoblotted for JIP1 and PARP as indicated. Similar results were obtained with staurosporine (not shown).