Fig. 5.

Identification of caspase-3 cleavage sites in JIP1. (A) Sequence alignment showing conserved caspase-3 cleavage sites in JIP1 from various species. (B) A diagram of JIP1 indicating the proposed sites of caspase-3 cleavage and the sizes of the expected cleavage products. Also shown are the positions of the JNK binding domain (JBD), Src homology 3 domain (SH3) and phosphotyrosine binding domain (PTB). (B) Wild-type and cleavage-resistant JIP1 mutants DLIA, DESA and the double mutant DLIA/DESA were ³⁵S-labelled by in vitro translation and incubated with or without 10 nM caspase-3 for 1 h at 30 °C. The reaction products were visualised by SDS-PAGE and autoradiography.