Fig. 6.

Effect of caspase-3 inhibition on JNK activation by TRAIL and staurosporine in HeLa cells. HeLa cells were treated with (closed symbols; ●, ▲) or without (open symbols; ○, △) 1 μg/ml TRAIL (A) or 1 μM staurosporine (B) in the presence (triangles; △, ▲) or absence (circles; ○, ●) of 40 μM DEVD.fmk. At the time points indicated, cell lysates were prepared from adherent and non-adherent cells, and JNK activity measured by immune-complex kinase assay as described in Experimental Procedures. The results shown represent the mean ± SEM for four independent experiments. Data points marked by asterisks (*) indicate statistically significant differences at p < 0.05 in a two-tailed t-test.