TABLE 2.

eSTPs in bacteria^a

Species	eSTP	Type	Partner kinase	Substrate	Function	Phospho-residue(s)	Methodology	Reference
Bacillus anthracis	Ba-Stp1	PP2C	Ba-Stk1	Ba-Stk1-P	eSTK	NA	In vitro phosphatase assay	161
Bacillus subtilis	PrpC	PP2C	PrkC	EF-G	Translation factor	NA	In vitro phosphatase assay	50
				CpG	Small ribosome-associated GTPase	NA	In vitro phosphatase assay	1
				EF-Tu	Translation factor	NA	In vitro phosphatase assay	1
				HPr kinase	Phosphotransferase system	S46	In vitro phosphatase assay	169
				PrkC-P	eSTK	NA	In vitro phosphatase assay	129
				YezB	Stress response	NA	In vitro phosphatase assay	1
	SpoIIE	PPM		SpoIIAA	Phosphoprotein anti-anti-sigma factor	NA	In vitro phosphatase assay	127
	RsbU	PPM	RsbV-P	RsbV-P	Regulation of sigma B	NA	In vitro phosphatase assay	193
	RsbX	PPM	RsbS-P	RsbS	Regulation of sigma B	NA	In vitro phosphatase assay	193
Listeria monocytogenes	Stp	PPM		EF-Tu	Translation factor	NA	2D phosphoprotein gel electrophoresis, in vitro phosphatase assay, decreased kirromycin sensitivity	5
Mycobacterium tuberculosis	PstP	PPM	PknB	PBPA	PBP, cell wall	NA	In vitro phosphatase assay	32
				PknB-P	eSTK	T171/T173	Mass spectrometry	18
Mycoplasma pneumoniae	PrpC	PP2C		HPr	Phosphocarrier protein of phosphotransfer system	S46	In vitro phosphatase assay	61
Myxococcus xanthus	Pph1	PP2C	Pkn5	Pkn5	Negative effector of development	NA	Yeast two-hybrid interaction	180
Staphylococcus aureus	Stp1	PP2C	Stk1	Stk1-P	eSTK	NA	In vitro phosphatase assay	16
Streptococcus agalactiae	SaSTP	PP2C		PpaC	Family II inorganic pyrophosphatase		In vitro phosphatase assay	144
				PurA	Purine biosynthesis	NA	In vitro phosphatase assay
Streptococcus pneumoniae	PhpP	PP2C	StkP	StkP-P	eSTK		Coimmunoprecipitation	131
				RitR	Transcriptional regulator		In vitro phosphatase assay	184
Streptococcus pyogenes	SP-STP	PP2C	SP-STK	SP-HLP	Histone-like protein	Thr(s)	In vitro phosphatase assay	72
							Phospho-amino acid analysis
				SP-STK-P	eSTK	NA	In vitro phosphatase assay	72

^aNA, not available.