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. 2001 Mar 13;98(6):3220–3225. doi: 10.1073/pnas.051633398

Figure 3.

Figure 3

Isolation of complexes between D-AKAP2 and RII from mouse brain extract. cAMP pull-down assays were performed as described in Experimental Procedures. Lanes 1–3 are: total homogenate, supernatant, and resuspended pellet (a small fraction of the whole sample loaded). Lanes 4–6 and 7–9 are: flow through, cAMP elute and sample buffer elute after binding to the resin in the absence (lanes 4–6) or presence (lanes 7–9) of cAMP (entire amount of sample loaded). Lane 10 has purified RII standards.