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. Author manuscript; available in PMC: 2012 Mar 9.

Published in final edited form as: Vaccine. 2011 Jan 28;29(12):2262–2271. doi: 10.1016/j.vaccine.2011.01.040

A Flow cytometric analysis after 24h incubation without rSm-p80 (Mock) or with rSm-p80 stimulation. CD3 membrane marker gating is used to see IFN-γ production from CD3 positive CD8 T cells. Intracellular secretion of IFN-γ in splenocytes of control (ODN immunized) and experimental (rSm-p80 + ODN immunized) groups of both knockout (µMt−/−; B10.129S2 (B6)-Igh-6^tm1Cgn/J) (KoC) and normal C57BL/6 (NC) mice.

B Flow cytometric analysis after 24h incubation without rSm-p80 (Mock) or with rSm-p80 stimulation. CD3 membrane marker gating is used to see IL-4 production from CD3 positive CD8 T cells. Intracellular secretion of IL-4 in splenocytes of control (ODN immunized) and experimental (rSm-p80 + ODN immunized) groups of both knockout (µMt−/−; B10.129S2 (B6)-Igh-6^tm1Cgn/J) (KoE) and normal C57BL/6 (NE) mice.

A Flow cytometric analysis after 24h incubation without rSm-p80 (Mock) or with rSm-p80 stimulation. CD3 membrane marker gating is used to see IFN-γ production from CD3 positive CD8 T cells. Intracellular secretion of IFN-γ in splenocytes of control (ODN immunized) and experimental (rSm-p80 + ODN immunized) groups of both knockout (µMt−/−; B10.129S2 (B6)-Igh-6^tm1Cgn/J) (KoC) and normal C57BL/6 (NC) mice.

B Flow cytometric analysis after 24h incubation without rSm-p80 (Mock) or with rSm-p80 stimulation. CD3 membrane marker gating is used to see IL-4 production from CD3 positive CD8 T cells. Intracellular secretion of IL-4 in splenocytes of control (ODN immunized) and experimental (rSm-p80 + ODN immunized) groups of both knockout (µMt−/−; B10.129S2 (B6)-Igh-6^tm1Cgn/J) (KoE) and normal C57BL/6 (NE) mice.