Table 3.
The level of cytokine production of Sm-p80 stimulated splenocytes of (homologous and heterologous transfer mice) and (µMt−/−; B10.129S2 (B6)-Igh-6tm1Cgn/J) knockout and normal C57BL/6 mice after rSm-p80 stimulation in vitro for 24 hours; values are given in pg/ml (Mean±SD).
| Groups (Transfer/Immunization) |
n | IL-2 (pg/ml) | IL-4 (pg/ml) | IL-10 (pg/ml) | INF-γ (pg/ml) |
|---|---|---|---|---|---|
| HoC (Sera) | 10 | 4.4±0.8 | 2.1±0.0 | 3.1±0.8 | 98.2±1.0 |
| HoE (Sera) | 10 | 4.7±0.2 | 2.3±0.1 | 3.3±1.2 | 228.6±1.1** |
| HeC (IgG) | 20 | 4.2±0.0 | 2.2±0.0 | 3.2±1.2 | 98.2±1.1 |
| HeE (IgG) | 35 | 4.5±0.0 | 2.2±0.1 | 3.3±0.8 | 125.3±4.4* |
| KoC (ODN) | 9 | 5.94±0.73 | 9.81±2.59 | 38.96±6.31 | 111.1±23.57 |
| KoE (ODN + rSm-p80) | 9 | 23.25±5.89* | 11.66±0.93€ | 51.10±0.88€ | 258.13±7.39* |
| NC (ODN) | 10 | 12.71±3.58 | 8.30±0.47 | 39.46±1.17 | 103.70±15.71 |
| NE (ODN + rSm-p80) | 10 | 19.40±5.67* | 12.00±0.47€ | 58.00±1.75€ | 278.14±8.32* |
The given codes: HoC, Homologous Control; HoE, Homologous Experimental; HeC, Heterologous Control; HeE, Heterologous Experimental; NC, Normal C57BL/6 control; NE, Normal Experimental; KoC and KoE are Knockout control and experimental C57BL/6 mice respectively. KoC and NC were immunized with ODN, but KoE and NE were immunized with rSm-p80 plus ODN; ODN, oligodeoxynucleotides;
**
p < 0.001;
*
p < 0.01;
€
p > 0.05