A, Domain structures of rescue proteins.
B-E, Sample traces and quantitative analysis of Ca2+-dependence of release of IPSCs in control neurons, cDKO neurons, and cDKO neurons rescued with the PDZ-domain deficient RIM-ΔPDZ fragment. Absolute IPSC amplitudes (C), IPSC amplitudes normalized to the response at 10 mM [Ca2+]ex (D), and apparent Ca2+-affinities (EC50 values; E) are indicated (control, n=10/3; cDKO, n=9/3: cDKO+RIM-ΔPDZ, n= 10/3).
F-H, Speed and synchrony of neurotransmitter release in control neurons, cDKO neurons and cDKO neurons rescued with RIM-ΔPDZ (control, n=7/3; cDKO, n=10/3: cDKO+RIM-ΔPDZ, n=12/3).
I and J, Sample line scans (I) and summary data (J) of action potential evoked Ca2+-transients in presynaptic boutons of control neurons, cDKO neurons and cDKO neurons rescued with RIM1α or RIM-ΔPDZ. Data shown are means (line) ± SEMs (shaded area). (boutons: control, n=40 boutons/6 neurons/5 independent cultures; cDKO, n=57/7/5; cDKO+RIM1α, n=51/7/5, cDKO+RIM-ΔPDZ, n=52/7/5; dendrites: control, n=22/6/5; cDKO, n=22/7/5; cDKO+RIM1α, n=19/7/5, cDKO+RIM-ΔPDZ, n=22/7/5). For cumulative peak amplitudes and statistical values, see Fig. S7 and Table S6).
Statistical analyses: *, p<0.05; **, p<0.01; ***, p<0.001; E, G and H, one-way ANOVA ; J, two-way ANOVA for peak amplitudes during the first 60 ms after action potential induction.