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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Phytopathol Mediterr. 2009 Aug;48(1):59–72.

Table 3.

Differences between the DNA-extraction protocols tested. Varied concentrations (w/v) of CTAB, SDS and skim milk used in extraction buffers are indicated.

Method CTAB (%) SDS (%) Skim milk (%) Time (d) Additional treatment
1 2 2 Proteinase K
2 2 2 2 Proteinase K
3 0.02 1
4 0.02 10 1
5 10 1 Incubation (65°C) before homogenisation
6 10 1 Incubation (65°C) after homogenisation
7 10 1
8 10 2 1
9 2 2
10 10 2 1
11 10 2 1 Sodium-acetate + ethanol combined
12 2 2 1 Sodium-acetate + ethanol combined