A. Stereotypical chromatin structure of most yeast promoters.
B. Spo11 oligos cluster in promoter NDRs. Normalized nucleosome occupancy is the number of sequence reads covering a base pair divided by genome average coverage (Kaplan et al., 2009).
C. Genome average nucleosome occupancy and Spo11 oligo density around all annotated TSS and separately for TATA-containing and TATA-less genes.
D. Hotspots are nucleosome depleted (n=3600, omitting exogenous hotspots).
E. DSB activity is not correlated with degree of nucleosome depletion at hotspot centers. Hotspots were divided into quintiles by oligo count.
F. Low nucleosome occupancy is not sufficient for strong DSB activity. Mean profiles are shown for 3′ ends of convergent genes.
Spo11 oligos in C, D, F were smoothed with a 75 bp Hann window.