Table 3. Metal content analysis of wild type FeCBS and CoCBS using ICP-OES.

For the CoCBS samples, 7× and 12× denotes number of cell growth passages in minimal medium supplemented with 150 μM CoCl₂ prior induction of CBS expression. Metal content is reported as percentage of total metal content.

Sample	Iron content (μM)	Iron content (%)	Cobalt content (μM)	Cobalt content (%)
WT FeCBS	1.3 ± 0.1	97.1 ± 0.7	0.036 ± 0.006	2.9 ± 0.2
CoCBS 7×	0.061 ± 0.002	13.1 ± 0.1	0.39 ± 0.04	86.7 ± 0.3
CoCBS 12×	0.083 ± 0.003	7.6 ± 0.4	0.96 ± 0.06	92.4 ± 0.8