Figure 7. The Scorecard Enables Quick and Comprehensive Characterization of Human Pluripotent Cell Lines.

(A) Schematic illustration of the similarity between ES and iPS cell lines in the epigenetic and transcriptional space. The density plot on the left depicts the variation observed among human ES cells. The two crosses indicate the (hypothetical) average of all ES and iPS cell lines, which this study approximated by profiling 20 human ES cell lines and 12 human iPS cell lines. The scatterplot on the right simulates the distribution of a large number of human iPS cell lines, taking into account their moderately increased variation (Figure 3B) as well as the observation that a minority of iPS cell lines were indistinguishable from ES cell lines (Figure 3D). Gaussians were used to simulate the ES cell and iPS cell distribution in silico.

(B) Outline of a workflow for high-throughput characterization of human pluripotent cell lines. Cell line characterization is performed in an iterative fashion, starting with the quantitative differentiation assay and performing additional characterizations only on those cell lines that the lineage scorecard identifies as useful for the application of interest.