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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Bioconjug Chem. 2011 Feb 21;22(3):445–454. doi: 10.1021/bc1004813

Figure 5.

Figure 5

In vivo PD study of various PEGylated TLR7 ligand conjugates. The in vivo kinetics of proinflammatory cytokine induction by PEGylated TLR7 ligands were determined after intravenous injection of C57BL/6 mice (n = 5 per group) with 200 nmol unconjugated or PEGylated TLR7 ligand conjugates. Serum samples were collected 2, 4, 6, 24, and 48 hours after injection. The levels of TNFa (A) and IL-6 (B) were measured by Luminex assay. Data are means ± SEM of five mice and are representative of two independent experiments. * and + denote p< 0.05 compared to the vehicle-treated and 1V136-treated mice, respectively, by one-way ANOVA tests with Bonferroni post hoc testing.