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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Jan 18;177(1):35–41. doi: 10.1016/j.molbiopara.2011.01.006

Fig. 2. Expression of SmDAT in mammalian COS-7 cells.

Fig. 2

A: Dopamine, norepinephrine, and serotonin uptake by human DAT, NET and SmDAT. Uptake was assayed at a concentration of 100 nM for each substrate and normalized to the dopamine uptake level. B: Kinetic analysis of dopamine uptake in COS-7 cells expressing hDAT (■), hNET (▲), or GFP-SmDAT (▼). Shown here is a representative experiment using twelve substrate concentrations with two replicates. Data from four independent experiments generated values of 4.2 ± 0.5 µM for the KM and 0.9 ± 0.2 pmol/well/min for the Vmax for hDAT; 0.47 ± 0.08 µM for the KM and 0.17 ± 0.03 pmol/min for the Vmax for hNET and 2.0 ± 0.3 µM for the KM and 0.16 ± 0.04 pmol/min for the Vmax for GFP-SmDAT. Values are given as Mean ± SEM. Non-specific uptake was determined by parallel uptake in mock-transfected cells and subtracted.