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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Jan 18;177(1):35–41. doi: 10.1016/j.molbiopara.2011.01.006

Fig. 3. SmDAT expression in the parasitic lifestages of the parasite developmental cycle.

Fig. 3

RT-PCR assessing SmDAT (Top panel) and Smα-tubulin (Bottom panel) mRNA expression throughout different developmental stages of the S. mansoni life cycle. Approximately 3 µg of total RNA from adult worms, schistosomula, eggs, cercariae, miracidium, infected snails (sporocysts) and non infected snails (control without infection), were reverse transcribed and amplified by PCR using SmDAT and tubulin specific primers.
  1. MW – 100bp DNA ladder
  2. Non-infected snail (control without infection)
  3. Infected snail 45 days (sporocysts)
  4. Cercariae
  5. Schistosomula (24 hours)
  6. Adult worms
  7. Eggs
  8. Miracidium