Table 1. Inhibitory constants (IC₅₀) for transport inhibitors in hDAT, hNET, and GFP-SmDAT.

Transiently transfected COS-7 cells were incubated for 10 min with increasing concentrations of the indicated inhibitor, followed by the addition of [³H]DA to give a final concentration of 50 nM [³H]DA. Putative substrates were not pre-incubated but instead incubated directly with 50 nM [³H]DA. The uptake was allowed to continue for 10 min at 20°C before washing. IC₅₀ values were calculated from at least five experiments performed in duplicate. Values are given in µM and represent Mean ± SEM.

	Human DAT	Human NET	GFP-SmDAT
Norepinephrine	9.80±1.50	1.87±0.30	17.8±1.8
d-Amphetamine	0.251±0.049	0.0670±0.0100	10.0±0.6
p-Tyramine	4.12±0.81	1.03±0.40	46.3±4.6
b-Phenethylamine	2.74±0.44	0.385±0.053	42.5±1.4
Cocaine	0.292±0.036	0.714±0.095	29.4±3.9
Benztropine	0.509±0.070	2.05±0.18	21.6±1.5
Bupropion	1.06±0.22	9.21±1.19	242±36
Nisoxetine	0.862±0.084	0.00146±0.00065	0.114±0.010
Desipramine	42.9±6.17	0.0281±0.0034	1.41±0.16
GBR-12909	0.00244±0.00137	0.743±0.168	3.08±0.50
Citalopram	157±25	17.1±4.1	50.5±4.7