Table 1.
Effect of ICV or IP injection of kisspeptin-10 on 4-h meal pattern.
| Parameter | Fed ad libitum in dark phase | Fed ad libitum in light phase | Overnight fasted/re-fed in light phase |
|||
|---|---|---|---|---|---|---|
| Vehicle (ICV) |
Kisspeptin-10 (1 µg, ICV) |
Vehicle (ICV) |
Kisspeptin-10 (1 µg, ICV) |
Vehicle (IP) |
Kisspeptin-10 (10 µg, IP) |
|
| Food intake/4h (g) | 0.47 ± 0.08 | 0.48 ± 0.10 | 0.04 ± 0.02 | 0.11 ± 0.03 | 1.25 ± 0.06 | 1.16 ± 0.05 |
| Meal frequency (number/4h) |
4.57 ± 1.00 | 5.29 ± 1.04 | 1.25 ± 0.48 | 1.75 ± 0.48 | 1.43 ± 0.20 | 1.63 ± 0.26 |
| Bout frequency (number/4h) |
17.00 ± 2.31 | 18.71 ± 1.69 | 4.00 ± 0.82 | 7.25 ± 2.59 | 24.00 ± 2.37 | 21.00 ± 1.56 |
| Meal size (g/meal) | 0.11 ± 0.02 | 0.09 ± 0.01 | 0.04 ± 0.01 | 0.06 ± 0.00 | 0.98 ± 0.13 | 0.82 ± 0.11 |
| Meal duration (min) |
12.13 ±2.51 | 9.64 ± 1.13 | 4.02 ± 2.88 | 7.06 ± 2.22 | 117.49 ±19.72 | 109.02 ± 18.06 |
| Total meal time (min/4h) |
48.78 ± 8.66 | 47.81 ± 9.19 | 4.87 ± 3.80 | 13.82 ± 5.75 | 147.66 ± 11.67 | 147.83 ± 10.24 |
| Time spent in meals (%/4h) |
20.30 ± 3.59 | 19.92 ± 3.83 | 1.68 ± 1.20 | 5.76 ± 2.40 | 61.52 ± 4.86 | 61.59 ± 4.27 |
| Latency to 1st meal (min) |
40.89 ± 23.62 | 43.71 ± 27.12 | 22.66 ± 13.22 | 43.23 ± 31.66 | 5.66 ± 1.21 | 4.65 ± 1.73 |
| Duration 1st meal (min) |
23.95 ± 4.49 | 15.10 ± 2.48 | 4.86 ± 2.70 | 6.75 ± 1.77 | 135.27 ± 14.50 | 110.54 ± 17.27 |
| Inter-meal interval (min) |
73.01 ± 21.00 | 48.73 ± 10.32 | 183.10 ± 94.97 | 176.46 ± 84.29 | 111.40 ± 13.83 | 94.57 ± 8.94 |
| Eating rate/4h (mg/min) |
2.33 ± 0.44 | 2.60 ± 0.45 | 0.71 ± 0.59 | 0.64 ± 0.29 | 4.32 ± 0.27 | 4.39 ± 0.34 |
| Satiety ratio (min/g) |
875.61 ± 424.19 | 572.51 ± 139.90 | 2714.58 ± 2396.25 | 2270.11 ± 1230.59 | 117.95 ± 11.59 | 124.52 ± 15.57 |
Each group had vehicle controls tested at the same time of kisspeptin-10 also injected ICV or IP. Data are mean ± SEM; n=6–8 mice/group.