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. 2011 Jan 5;4(2):300–309. doi: 10.1093/mp/ssq076

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© The Author 2011. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The Expression of GUS Driven by Native (P_Xa13 and P_xa13) or Truncated (P_Xa13-934, P_Xa13-394, P_xa13-1147, and P_xa13-608) Promoters after PXO99-Inoculation or Mock-Inoculation at the Booting Stage.

GUS activity was determined by measuring the amount of 4-methylumbelliferone (Mu) produced under the catalysis of GUS in 1 mg total protein per minute. Sixteen to 22 T₀ transgenic plants carrying each construct were used for analyses. For each time point examined, leaf fragments were collected from all the plants carrying the same construct and mixed to prepare a sample. The GUS activity at each time point was the average of three measurements ± standard deviation. The ‘a’ indicates that a significant difference (P < 0.01) was detected between pathogen-inoculated and mock-inoculated plants in the same time point.