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. 2011 Jan 5;4(2):300–309. doi: 10.1093/mp/ssq076

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© The Author 2011. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Expression of GUS Driven by Native (P_Xa13) or Mutated (P_Xa13_Δ79T, P_Xa13_Δ75A, P_Xa13_Δ78A, and P_Xa13_Δ77G) Promoters after PXO99 Infection or Mock-Inoculation at the Booting Stage.

(A) The structures of mutated promoters and the sites of mutations. The UPT_PthXo1 box of P_Xa13 is shown with bold italic letters. The figure with a sign of substitution indicates the site of nucleotides substitution.

(B) Expression of GUS in transgenic plants. GUS activity was determined by measuring the amount of 4-methylumbelliferone (Mu) produced under the catalysis of GUS in 1 mg total protein per minute. Twenty to 36 T₀ transgenic plants carrying each construct were used for analyses. For each time point examined, leaf fragments were collected from all the plants carrying the same construct and mixed for preparing sample. The GUS activity at each time point was the average of three measurements ± standard deviation. ck, without pathogen- or mock-inoculation. The asterisk (*) indicates that a significant difference (P < 0.01) was detected between PXO99-inoculated plants and non-inoculated control (ck) plants carrying the same promoter.