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. 2010 Jul 26;108(Suppl 1):4615–4622. doi: 10.1073/pnas.1000082107

Fig. 2.

Fig. 2.

Inflammation is attenuated in GF mice on EAE induction. (A) Lymphocytes were harvested from draining LN of treated SPF and GF mice at 8 d after immunization with MOG/CFA. Intracellular staining of CD4+ T cell for IFNγ and IL-17A after 3 d in vitro culture with MOG peptide, restimulated with phorbol 12-myristate 13-acetate (PMA)/ionomycin for the last 5 h. Numbers in each quadrant indicate percentage of cytokine-positive CD4+ T cells. (B) Mean ± SD of the CD4+IFNγ+ T cell subsets from A. Data are representative of three independent experiments with at least four mice per group. (C) Real-time PCR of the transcription factor RORγt in cells isolated by Percoll gradient from spinal cords of SPF and GF mice at the onset of disease (day 8). Each symbol represents a single mouse. (D) IFNγ and IL-17A cytokine ELISA from cells harvested from draining LN of SPF and GF mice 8 d after immunization with MOG/CFA and cultured for 3 d in vitro with MOG peptide. Data are representative of three independent experiments, with mean ± SD of samples run in triplicate.