Fig. 3.

Increase in CD25⁺Foxp3⁺ Treg cells after induction of EAE in GF mice. Intracellular staining of CD4⁺CD25⁺Foxp3⁺ T cells cultured for 3 d with MOG peptide, restimulated with PMA/ionomycin for the last 5 h. Lymphocytes were harvested from draining lymph nodes (LN) and spleens of SPF and GF mice at 8 d p.i. (A and B) or 15 d p.i. (C and D) after i.v. immunization with MOG/CFA. In A and C, numbers in each quadrant indicate percentage of positive cells. In B and D, results are shown for mean ± SD of the CD4⁺ subsets of the cells from draining LN and spleen on day 8 and day 15 postimmunization. Data are representative of three independent experiments with at least four mice per group.